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Membrane Compartmentalisation and Cell Polarity Lab

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DESCRIPTION OF LAB RESEARCH WORK

We are interested in the molecular, structural, and functional organisation of the cell membrane. Our overarching goal is to understand how compartmentalization of the cell cortex regulates cellular form and function and complex cell behaviours such as cell migration and polarity, in particular in epithelial cells. We combine cell biological, biochemical, structural and functional approaches with highresolution imaging including light and electron microscopy and correlative imaging.

LAB MEMBERS
LEAD PI
Alexander Ludwig
Assistant Professor

Email: aludwig@ntu.edu.sg
Phone: (65) 6908 2212
Office: EMB-06-09
Lab webpage
Andra Sujana
Research Associate

Email: sujana.andra@ntu.edu.sg
Phone: (65) 6908 2213
Barbara Huebner
Research Associate

Email: bhuebner@ntu.edu.sg
Phone: (65) 6908 2213
Siti Maryam Jameelah Binte Mohd Yatim
Research Associate

Email: siti.maryam@ntu.edu.sg​
Phone:(65) 6908 2213
Hana Maldivita Tambrin
PhD student

Email: Hana0008@e.ntu.edu.sg
Phone: (65) 6908 2213
Sujasha Ghosh
PhD student

Email: sujasha001@e.ntu.edu.sg
Phone: (65) 6908 2213


CURRENT PROJECTS
  • Nanometer-scale organisation of the epithelial cell cortex

  • Functional characterisation of novel polarity regulators in mammalian epithelial cells

  • Correlative light and electron microscopy of membrane architecture



PUBLICATIONS
Full list of publications can be found here​
  • Tan, B, Peng, S, Yatim, SMJM, Gunaratne, J, Hunziker, W, and Ludwig, A#. An optimised protocol for proximity biotinylation in confluent epithelial cell cultures using the peroxidase APEX2. STAR Protocols. Published July 31 2020 doi: https://doi.org/10.1016/j.xpro.2020.100074. #corresponding author

  • Tan, B, Yatim, SMJM, Peng, S, Gunaratne, J, Hunziker, W, and Ludwig, A#. The mammalian Crumbs complex defines a distinct polarity domain apical of epithelial tight junctions. Current Biology. Published June 11 2020. doi: https://doi.org/10.1016/j.cub.2020.05.032. #corresponding author

  • Ludwig, A#. Selective Visualization of caveolae by TEM using APEX2. Caveolae - Methods and Protocols. Methods in Molecular Biology. 2020;2169:1-10. doi: https://doi.org/10.1007/978-1-0716-0732-9. #corresponding author

  • Tie, HC, Ludwig, A, Sandin, S, Lu, L. The Spatial Separation of Processing and Transport Functions to the Interior and Periphery of the Golgi Stack. Elife. 2018 Nov 30;7. pii: e41301. doi: https://doi.org/10.7554/eLife.41301.

  • Ludwig, A#, Nguyen, TH, Leong, D, Ravi, LI, Huan, TB, Sandin, S & Sugrue, R#. 2017. Caveolae provide a specialized membrane environment for respiratory syncytial virus assembly. J Cell Sci. 2017 Feb 2. doi: https://doi.org/10.1242/jcs.198853. #corresponding author.

  • Ludwig, A#, Nichols, BJ, Sandin, S. Architecture of the Caveolar Coat Complex, J Cell Sci. 2016 Jul, doi: https://doi.org/10.1242/jcs.191262. #corresponding author.

  • Shvetz E, Ludwig A, Nichols BJ. News from the caves: update on the structure and function of caveolae. Curr. Opin. Cell Biol. 2014 Jun 5; 29C:99-106. doi: https://doi.org/10.1016/j.ceb.2014.04.011.

  • Ludwig A#, Howard G, Mendoza-Topaz C, Deerinck T, Mackey M, Sandin S, Ellisman, MH, and Nichols B#. Molecular Composition and Ultrastructure of the Caveolar Coat Complex. PLoS Biology. 2013 Aug;11 (8), e1001640. Completed doi: https://doi.org/10.1371/journal.pbio.1001640. #corresponding author.

  • Ludwig A*, Otto GP*, Riento K, Hams E, Fallon PG, Nichols BJ. Flotillin microdomains interact with the cortical cytoskeleton to control uropod formation and neutrophil recruitment. J Cell Biol. 2010 Nov;15;191(4):771-81. doi: https://doi.org/10.1083/jcb.201005140. *equal contribution.

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